For citation:
Naumova M. N., Meshherjakova M. O., Turkovskaja O. V., Kazarinov I. A. Icomparative studying of kinetics of bioelectrochemical oxidation of glucose in neutral environments by means of the microorganisms Escherichia coli and Enterobacter cloacae. Electrochemical Energetics, 2015, vol. 15, iss. 3, pp. 130-135. DOI: 10.18500/1608-4039-2015-15-3-130-135, EDN: WHOUQF
Icomparative studying of kinetics of bioelectrochemical oxidation of glucose in neutral environments by means of the microorganisms Escherichia coli and Enterobacter cloacae
The method of the rotating disk electrode studied electrochemical behavior redox mediator methylene blue in bioelectrochemical system «glucose – cell – mediator – electrode» and is shown that methylene blue is reversible oxidation-reduction a mediator and can be applicable at realization of the microbic media even anode on the basis of glucose and cell of Escherichia coli and Enterobacter cloacae.
It is established that the limiting stage of process of bioelectrochemical oxidation of glucose in neutral environments is diffusion reduce to condition a mediator through a cellular membrane of a microorganism. It is shown that the size of its coefficient of diffusion through a cellular membrane of microorganisms of E. coli makes (4.5± 0.2)•10–7 cm2/s with that diffusion coefficient sizes through a cellular membrane of microorganisms of En. cloacae (1.3±0.3)•10–7 of cm2/s are 3.5 times higher with and, therefore, cell of E. coli are more effective biocatalyst the process of oxidation of glucose.
1. Davila D., Esquivel J., Vigues N. Development and Optimization of Microbial Fuel Cells. J. New Mater. Electroch. Systems, 2008, vol. 11, pp. 99–103.
2. Miller D. Eksperimenty v molekulyarnoy genetike. [Experiments in molecular genetics]. Moscow, Mir Publ., 1976, pp. 440 (in Russian).
3. Lurie Y. Y. Spravochnik po analiticheskoy khimii. [Handbook of Analytical Chemistry]. Moscow, Khimiya Publ., 1965, pp. 390 (in Russian).
4. Kazarinov I. A., Ignatova A. A., Naumova M. N. Kinetics of the Electrocatalytic Oxidation of Glucose by Escherichia coli Bacterial Cells in the Presence of Exogenous Mediators. Russian J. Electrochemistry, 2014, vol. 50, no. 1. pp. 87–91 (in\linebreak Russian).
5. Gradskij D. A., Kazarinov I. A., Ignatov V. V. Bioelektrokhimicheskoye okisleniye glyukozy s pomoshch'yu bakterii Escherihia coli [Bioelectrochemical glucose oxidation using bacteria Escherihia coli]. Russian J. Electrochemistry, 2000, vol. 37, no. 6, pp. 1397–1400 (in Russian).
6. Kim J. R., Jung S. H., Regan J. M., Logan B. E. Electricity generation and microbial community analysis of alcohol powered microbial fuel cells. Bioresource Technology, 2007, vol. 98, pp. 2568–2577.
7. Pleskov Y. V., Filinovskiy V. Y. Vrashchayushchiysya diskovyy elektrod [Rotating disk electrode]. Moscow, Nauka Publ., 1972, 344 p. (in Russian).
8. Varfolomeyev S. D. Khimicheskaya enzimologiya [Chemical enzymology]. Moscow, Akademiya Publ., 2005, 480 p. (in Russian).
9. Kazarinov I. A., Kuzmicheva E. V., Ignatova A. A. The estimation of performance of exogenous redox mediatorsin the bioelectrochemical system glucose – Escherihia coli cells – mediator. Elektrokhimicheskaya energetika (Electrochemical energetics), 2011, vol. 11, no. 2. pp. 60–64.